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包装
pack of 1 × 25 pieces
メーカー／製品名
Cytiva 28-9042-67
詳細
PlasmidPrep Midi Flow Kit is a gravity flow plasmid prep kit designed for the purification of large yields of transfection-grade plasmid DNA that is proportional to the input sample volume.
The mechanism of adding a DNA plasmid into a mammalian cell is known as plasmid transfection, and for it to work, transfection-grade plasmid DNA is needed. To obtain efficient gene transfer by transfection, plasmid DNA can be complexed with lipid reagents for more efficient delivery into the cell′s nucleus, essential for subsequent protein expression of the gene of interest.
PlasmidPrep Midi Flow Kit can be used to process bacterial sample volumes of 25 to 50 mL for high copy number plasmid DNA and up to 500 mL for low copy number plasmid DNA. The kit consists of a high-performance, anion exchange purification medium that combines excellent flow characteristics with an exceptional capacity and specificity for plasmid DNA.
PlasmidPrep Midi Flow Kit contains 250 fast-flow plasmid columns, resuspension, lysis, neutralization, wash, elution, and Tris-EDTA (TE) buffers, and a full protocol booklet with a detachable, quick reference protocol card.
The mechanism of adding a DNA plasmid into a mammalian cell is known as plasmid transfection, and for it to work, transfection-grade plasmid DNA is needed. To obtain efficient gene transfer by transfection, plasmid DNA can be complexed with lipid reagents for more efficient delivery into the cell′s nucleus, essential for subsequent protein expression of the gene of interest.
PlasmidPrep Midi Flow Kit can be used to process bacterial sample volumes of 25 to 50 mL for high copy number plasmid DNA and up to 500 mL for low copy number plasmid DNA. The kit consists of a high-performance, anion exchange purification medium that combines excellent flow characteristics with an exceptional capacity and specificity for plasmid DNA.
PlasmidPrep Midi Flow Kit contains 250 fast-flow plasmid columns, resuspension, lysis, neutralization, wash, elution, and Tris-EDTA (TE) buffers, and a full protocol booklet with a detachable, quick reference protocol card.
特徴および利点
- Provides plasmid DNA with excellent purity, > 70% (strain-dependent) of supercoiled plasmid DNA
- Increased ease of use due to minimal pipetting volume changes, color-coded kit components, and pre-equilibrated columns
- Increased ease of use due to minimal pipetting volume changes, color-coded kit components, and pre-equilibrated columns.
- Consistently produces pure plasmid DNA with low levels of endotoxin contamination, < 10 endotoxin units (EU) per μg of plasmid DNA
保管および安定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
アナリシスノート
この製品の分析証明書は、www.cytiva.com.
法的情報
illustra is a trademark of Cytiva
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
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資料
This page shows Plasmid DNA purification using illustra plasmidPrep Kits from GE Healthcare.
This page shows Plasmid DNA purification using illustra plasmidPrep Kits from GE Healthcare.
This page shows downstream applications for plasmid DNA and substances that may interfere in each.
This page shows possible causes and solutions for problems that may occur during plasmid DNA preparation.
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