GE28-4082-53

HiTrap^® MabSelect^™

Cytiva 28-4082-53, pack of 5 × 1 mL

• UNSPSCコード: 23151816
• NACRES: NA.56

特性

• ligand: recombinant protein A (E. coli)
• 包装: pack of 5 × 1 mL
• メーカー／製品名: Cytiva 28-4082-53
• 保管条件: (20% Ehtanol); (20% Ethanol)
• パラメーター: 42 psi
• ベッドサイズ: 7 mm × 25 mm
• ベッド容積: 1 mL
• カラム内径: 7 mm
• マトリックス: highly cross-linked agarose
• 平均部品サイズ: 85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
• cleaning: 2-12
• working range: 3-10
• キャパシティ: ≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)
• 適合性: suitable for bioprocess medium
• 保管温度: 2-8°C

詳細

詳細

Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.

HiTrap^® MabSelect^™ columns are prepacked with MabSelect, a Bioprocess medium for capuring of Mabs from large sample volumes.

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

アプリケーション

Lab Purification using the MabSelect^™ Protein A tag for capture of antibodies.

特徴および利点

• HiTrap^® columns prepacked with MabSelect, a Bioprocess medium for capuring of Mabs from large sample volumes. • The specificity of binding to the Fc region of IgG is similar to that of native protein A, which allows one-step purification.
• Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
• Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

その他情報

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法的情報

HiTrap is a registered trademark of Cytiva

MabSelect is a trademark of Cytiva

安全性情報

• ピクトグラム: GHS02
• シグナルワード: Warning
• 危険有害性情報: H226
• 注意書き: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• 保管分類コード: 3 - Flammable liquids