GE17-5268-02

Ni Sepharose^™ High Performance

Cytiva 17-5268-02, pack of 100 mL

• 別名: High Performance Ni Resin, Ni Sepharose
• UNSPSCコード: 41106500
• NACRES: NA.56

特性

• 包装: pack of 100 mL
• メーカー／製品名: Cytiva 17-5268-02
• マトリックス: highly cross-linked 6% agarose
• 平均部品サイズ: 34 μm
• cleaning in place: 2-14(Ni²⁺-stripped medium.)
• working range: 3-12(Ni²⁺-stripped medium.)
• キャパシティ: ≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)

詳細

詳細

Ni Sepharose^™ High Performance affinity media for high-resolution, high capacity purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Ni Sepharose^™ High Performance consists of highly cross-linked agarose beads to which a chelating group has been coupled. This chelating group is precharged with Ni²⁺, which will selectively retain proteins with exposed histidine groups.

The high stability and broad compatibility of Ni Sepharose^™ High Performance maintains biological activity and increases product yield, at the same time as it greatly expands the range of suitable operating conditions.

特徴および利点

• High-performance purification of histidine-tagged proteins
• Negligible leakage of Ni²⁺ ions.
• High binding capacity, at least 40 mg/mL medium
• Compatible with a wide range of reducing agents, detergents, denaturants and other additives
• 34 μm bead size for improved resolution

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

法的情報

IMAC Sepharose products, Ni Sepharose products and Fe Sepharose products
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.

Sepharose is a trademark of Cytiva

安全性情報

• ピクトグラム: GHS09
• シグナルワード: Warning
• 危険有害性情報: H226
• 注意書き: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• 保管分類コード: 3 - Flammable liquids