おすすめの製品
ligand
2-mercaptopyridine
包装
pack of 1 × 5 mL
メーカー/製品名
Cytiva 17-5111-01
保管条件
(20% Ethanol)
パラメーター
<20 mL/min flow rate
42 psi
ベッドサイズ
16 mm × 25 mm
ベッド容積
5 mL
カラム内径
16 mm
Matrix
highly cross-linked 6% agarose
平均部品サイズ
34 μm
cleaning
2-13
動作範囲
3-11
保管温度
2-30°C
関連するカテゴリー
詳細
HiTrap® IgY Purification HP is a 5 mL column prepacked with Sepharose™ High Performance for fast and easy purification of IgY from egg yolk.
アプリケーション
HiTrap® IgY Purification HP is packed with a thiophilic adsorption medium, 2-merCaptopyridine coupled to Sepharose™ High Performance. Thiophilic adsorption is promoted by water-structuring salts. It has been suggested that the interaction of IgY and ligand results from combined electron donating and accepting action of the ligand or, alternatively, a mixed mode hydrophilic-hydrophobic interaction.
特徴および利点
- Each column binds IgY from a one-quarter portion of egg yolk (approx. 100 mg IgY). For greater capacity, several columns may be connected in series.
- Packed with a thiophilic adsorption medium, the ligand, 2-merCaptopyridine is coupled to Sepharose™ High Performance.
- Recovery and purity of IgY are excellent.
- Can be operated with a liquid chromatography system such as ÄKTA® design.
- The top and bottom frits are manufactured from porous polyethylene
- Connectors for usage with different chromatography systems and other equipment are included
保管および安定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
アナリシスノート
この製品の分析証明書は、www.cytiva.com.
その他情報
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
法的情報
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
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資料
This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
プロトコル
This page shows how to prepare samples for purification with affinity chromatography.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら(テクニカルサービス)