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ligand
human IgG
包装
pack of 10 mL
メーカー/製品名
Cytiva 17-0969-01
保管条件
(20% Ethanol, 50 mM K2PO4, 1mM MgCl2 pH 7.2 in 20% Ethanol)
Matrix
highly cross-linked 6% agarose
平均直径
90 μm
cleaning in place
3-12
動作範囲
3-10
キャパシティ
~3 mg binding capacity(Protein A)
保管温度
2-8°C
詳細
lgG Sepharose™ 6 Fast Flow, from Cytiva, is based on the rigid Sepharose™ 6 Fast Flow matrix, with human IgG covalently coupled to it. The improved mechanical characteristics of this Fast Flow medium allows high flow rates to be used for rapid and convenient single step purification of protein A fusion protein conjungates produced in prokaryotic expression systems.
The mechanical characteristics of this fast flow medium allows high flow rates to be used for rapid and convenient single step purification of protein A fusion protein conjungates produced in prokaryotic expression systems.
特徴および利点
- IgG coupled to the highly cross-linked 6% agarose matrix Sepharose™ 6 Fast Flow, designed for the rapid purification of protein A and protein A fusion conjugates
- Binds at least 2 mg protein A/mL medium
- Suitable for tandem affinity purification (TAP) of protein complexes
保管および安定性
アナリシスノート
法的情報
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
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資料
This page shows how to perform a separation with IgG Sepharose 6 Fast Flow from GE Healthcare, an Affinity Chromatography product for purification of recombinant fusion proteins containing a protein A tail.
This page shows how to perform a separation with IgG Sepharose 6 Fast Flow from GE Healthcare, an Affinity Chromatography product for purification of recombinant fusion proteins containing a protein A tail.
This page shows the characteristics of Dextrin Sepharose High Performance products from GE Healthcare.
プロトコル
This page shows how to purify protein A-tagged proteins using GE Healthcare products.
This page shows how to purify protein A-tagged proteins using GE Healthcare products.
This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from GE Healthcare.
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from GE Healthcare.
関連コンテンツ
Pull-down assays, reagents, and protocols for investigating in vitro protein-protein interactions using affinity or GST pull-down, tandem affinity purification (TAP), and co-immunoprecipitation methods.
アフィニティーまたはGSTプルダウン法、タンデムアフィニティー精製(TAP)法、共免疫沈降法を用いてin vitroのタンパク質間相互作用を検討するためのプルダウンアッセイ、試薬、プロトコル
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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