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ligand
lentil lectin
包装
pkg of 25 mL
メーカー/製品名
Cytiva 17-0444-01
保管条件
(20% Ehtanol, PBS pH 7)
Matrix
4% agarose
粒径
45-165 μm
平均直径
90 μm
cleaning in place
3-12
動作範囲
3-10
保管温度
2-8°C
関連するカテゴリー
詳細
Lentil Lectin Sepharose™ media is used for purification of a number of glycoproteins and other carbohydrate containing molecules. The lentil lectin ligand has more equal affinity for α-D-mannose and α-D-glucose compared to Con A.
Lentil lectin is a metalloprotein, containing Ca2+ and Mn2+, which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars. Lentil Lectin Sepharose™ 4B is a generally applicable group specific adsorbent and is routinely used in the preparation and purification of a number of glycoproteins and carbohydrate containing molecules.
Lentil lectin is a metalloprotein, containing Ca2+ and Mn2+, which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars. Lentil Lectin Sepharose™ 4B is a generally applicable group specific adsorbent and is routinely used in the preparation and purification of a number of glycoproteins and carbohydrate containing molecules.
特徴および利点
- Binds molecules that contain α-D-mannose, α-D-glucose, and sterically related residues
- Compared to Con A, also for glycoprotein purification, Lentil Lectin distinguishes less sharply between glucosyl and mannosyl
- Retains binding in 1% deoxycholate and is especially valuable for purification of detergent-solubilized membrane proteins
- Lentil lectin is a metalloprotein, containing Ca2+ and Mn2+, which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars
保管および安定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
アナリシスノート
この製品の分析証明書は、www.cytiva.com.
法的情報
Sepharose is a trademark of Cytiva
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
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資料
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
プロトコル
Purification or Removal of Glycoproteins and Polysaccharides
Purification or Removal of Glycoproteins and Polysaccharides
Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.
Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.
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