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Merck

G9264

Sigma-Aldrich

モノクロナール抗成長関連タンパク質-43 マウス宿主抗体

clone GAP-7B10, ascites fluid

別名:

抗GAP-43抗体

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About This Item

MDL番号:
UNSPSCコード:
12352203
NACRES:
NA.41

由来生物

mouse

品質水準

結合体

unconjugated

抗体製品の状態

ascites fluid

抗体製品タイプ

primary antibodies

クローン

GAP-7B10, monoclonal

分子量

antigen 46 kDa

含みます

15 mM sodium azide

化学種の反応性

hamster, human, feline, rat, chicken, snake, mouse

テクニック

immunohistochemistry: suitable
western blot: 1:2,000 using newborn rat brain extract

アイソタイプ

IgG2a

UniProtアクセッション番号

輸送温度

dry ice

保管温度

−20°C

ターゲットの翻訳後修飾

unmodified

遺伝子情報

human ... GAP43(2596)
mouse ... Gap43(14432)
rat ... Gap43(29423)

詳細

Monoclonal Anti-Growth Associated Protein-43 (mouse IgG2a isotype) is derived from the GAP-7B10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with HPLC-purified GAP-43 from neonatal rat forebrain membranes. Growth Associated Protein 43 (GAP-43) has a very acidic pI (4.3-4.6, depending on the species) and an anomalous behavior in SDS gels such that its reported molecular mass ranges from 43-57 kDa whereas, the primary sequence of the protein and its hydrodynamic behavior indicate a molecular mass of about 24 kDa.

特異性

GAP-43のキナ-ゼCリン酸化型、脱リン酸化型の両方に存在するエピト-プを認識します。

免疫原

新生ラット前脳膜由来GAP-43。

アプリケーション

Monoclonal Anti-Growth Associated Protein-43 antibody produced in mouse has been used in enzyme linked immunosorbent assay (ELISA).
Monoclonal Anti-Growth Associated Protein-43 antibody produced in mouse is suitable for:
  • immunostaining of tissue sections from the prefrontal cortex and hippocampus of rat pups to recognize an epitope present on kinase C domain in the N terminal of GAP-43 protein
  • immunohistochemistry at a working dilution of 1:3000 using sections from fetal and adult brains of mice
  • immunofluorescence at a working dilution of 1:4000 using 8μm sections of mice brain
  • western blotting using hippocampal lysates from rats
It is also suitable for immunoblotting at a working dilution of 1:2000 using newborn rat brain extract. The antibody is useful in the study of the role and function of GAP-43 during axonogenesis and in the adult nervous system.

生物化学的/生理学的作用

Growth Associated Protein 43(GAP-43) precise function in growth cones and synaptic terminals is not known, increased phosphorylation of GAP-43 correlates with exocytosis, depolarization, and treatment of growth cones with NGF, implying that kinase C and possibly other protein kinase-mediated phosphorylation of GAP-43 could be a component of a common response of growth cones to diverse extracellular stimuli. GAP43 associates with the plasma membrane via acylated residues near the amino terminus and with the submembrane fraction of the cytoskeleton, presumably through its highly charged, extended carboxyl terminus, which bears similarities to neurofilament proteins. Monoclonal antibody reacting specifically against GAP-43 is a useful tool in the study of the role and function of GAP-43 during axonogenesis and in the adult nervous system.
The neural-specific Growth-Associated Protein-43 (GAP-43) is an intracellular phosphoprotein found exclusively in the peripheral and central nervous systems. The axonal growth cones that are involved in the functions of motility and pathfinding during axonogenesis and regeneration contain GAP-43. Phosphorylation of GAP-43 is associated with exocytosis, depolarization and treatment of growth cones with nerve growth factor (NGF).

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

10 - Combustible liquids

WGK

WGK 2

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

G9264-BULK:
G9264-.2ML:
G9264-VAR:
G9264-100UL:


試験成績書(COA)

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GAP-43--what does it do in the growth cone?
P R Gordon-Weeks
Trends in neurosciences, 12(10), 363-365 (1989-10-01)
Ming-Shu Xu et al.
Evidence-based complementary and alternative medicine : eCAM, 2013, 137631-137631 (2013-12-19)
Background. Cerebral ischemia is known to produce brain damage and related behavioural deficits, including memory deficits and motor disorders. Evidence shows that EA significantly promotes recovery of neurological function and thus improves quality of life. Objective. Evidence exists for the
K F Meiri et al.
Proceedings of the National Academy of Sciences of the United States of America, 83(10), 3537-3541 (1986-05-01)
Growth-associated protein, GAP-43, is a polypeptide that is induced in neurons when they grow axons. We show by means of subcellular fractionation and immunohistochemical localization that GAP-43 is a component of neuronal growth cones as well as growing neurites; it
A shift from a pivotal to supporting role for the growth-associated protein (GAP-43) in the coordination of axonal structural and functional plasticity
Holahan MR
Frontiers in Cellular Neuroscience, 11, 266-266 (2017)
Axonal growth-associated proteins.
J H Skene
Annual review of neuroscience, 12, 127-156 (1989-01-01)

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