AB5312

Anti-Growth Associated Protein 43 Antibody

serum, Chemicon^®

• 別名: GAP-43, Neuromodulin, B-50
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: serum
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 化学種の反応性: mouse, human, monkey, rat, feline, bovine
• メーカー／製品名: Chemicon^®
• テクニック: immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunoprecipitation (IP): suitable; western blot: suitable
• NCBIアクセッション番号: NM_002045.2
• UniProtアクセッション番号: P17677
• 輸送温度: dry ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... GAP43(2596)

詳細

詳細

GAP-43, a common marker of differentiating neurons, is expressed at elevated levels by developing or regenerating neurons during axon growth. While GAP-43 is an integral membrane protein associated with the cytoplasmic surface of axonal growth cones (and synapses), it is absent from dendritic growth cones. In adult brain, GAP-43 is found in high concentration in presynaptic areas where memory formation is thought to occur, such as frontal cortex, limbic system and hippocampus. Phosphorylation of GAP-43 by PKC (at Ser41 on human, mouse, rat, and bovine GAP-43 or Ser42 on chicken and Xenopus GAP-43) is specifically correlated with certain forms of synaptic plasticity.

特異性

AB5312 specifically recognizes GAP-43 regardless of the protein′s phosphorylation state. This antiserum has been refered to in the literature as CG-1.

免疫原

Purified GAP-43 from feline brain (McIntosh and Parkinson, 1990).

アプリケーション

Research Category
ニューロサイエンス

Research Sub Category
神経再生医学

神経シグナリング

Immunocytochemistry: 1:100-1:400 for mouse primary neurons, NTera2 neurons.

Immuohistochemistry: 1:2,000 on coronal sections of adult visual cortex (McIntosh and Parkinson, 1990). AB5312 has been used to stain GAP-43 in 4% paraformaldehyde-fixed paraffin-embedded tissue sections of mouse olfactory bulb (Legrier, 2001).

Western blot: 1:500 - 1:1,000. Detects a single band at approximately 50 kDa on western blots of protein extracts from adult visual cortex (McIntosh and Parkinson, 1990).

Immunoprecipitation

Optimal working dilutions must be determined by end user.

This Anti-Growth Associated Protein 43 Antibody is validated for use in IC, IH, IH(P), IP, WB for the detection of Growth Associated Protein 43.

ターゲットの説明

23.6 kDa

物理的形状

Unpurified

Serum. Liquid with 0.02% sodium azide added as a preservative.

保管および安定性

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

アナリシスノート

Control
Rat dorsal root ganglion tissue that has been subjected to a spinal nerve ligation, cultured neurons

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable