G6670

Anti-Growth Factor Independence-1 antibody, Mouse monoclonal

clone 2.5D.17, purified from hybridoma cell culture

• 別名: Anti-GFI1, Anti-ZNF163
• MDL番号: MFCD02263329
• UNSPSCコード: 12352203
• NACRES: NA.41
• 結合体: unconjugated
• application: ARR; IHC; WB
• クローン: 2.5D.17, monoclonal
• 化学種の反応性: rat, mouse, human
• citations: 11
• テクニック: immunohistochemistry: suitable; microarray: suitable; western blot: 1-2 μg/mL using total cell extract of A549 cells

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: primary antibodies
• クローン: 2.5D.17, monoclonal
• フォーム: buffered aqueous solution
• 分子量: antigen ~55 kDa
• 化学種の反応性: rat, mouse, human
• 濃度: ~2 mg/mL
• テクニック: immunohistochemistry: suitable; microarray: suitable; western blot: 1-2 μg/mL using total cell extract of A549 cells
• アイソタイプ: IgG1
• UniProtアクセッション番号: Q99684
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... GFI1(2672) ; mouse ... Gfi1(14581) ; rat ... Gfi1(24388)

詳細

詳細

Monoclonal Anti-Growth Factor Independence-1 (GFI1) (mouse IgG1 isotype) is derived from the hybridoma 2.5D.17 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with rat GFI1. Growth Factor Independence 1 (GFI1) is a zinc-finger transcriptional repressor, that is located on human chromosome 1p22. Gfi1 is highly expressed in thymus while Gfi1B is the predominant factor expressed in spleen, and both are expressed in bone marrow. It has a repression domain named SNAG at the N-terminal.† The protein also has a zinc finger DNA binding domain that is homologous to the domain in Gfi1B. Both bind to same consensus DNA sequence

特異性

抗体のエピト-プはGFI1 SNAGの転写抑制ドメイン内(アミノ酸7~26)に存在します。

免疫原

ラット成長因子非依存性-1タンパク質(GFI1)。

アプリケーション

Monoclonal Anti-Growth Factor Independence-1 (GFI1) antibody may be used in:

• immunoblotting
• immunohistochemistry
• EMSA (electrophoretic mobility shift analysis)

生物化学的／生理学的作用

Growth factor independence-1 (Gfi1) plays a key role in hematopoiesis along with Gfi1B proteins. Cytokines that regulate innate immunity induce Gfi1 expression. Deficiency of this protein causes thymic and peripherial T lymphopenia and severe abnormalities in pre-T-cell development in mice. Mutations in this gene cause defects in T lymphocyte function and development in humans. Gfi1 autoregulates its expression in T lymphocytes.

物理的形状

0.01 M PBS溶液(pH 7.4, 15 mMアジ化ナトリウム含有)。

アナリシスノート

抗体はEMSA(電気泳動移動度シフト解析)に使用することができます。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)