F1773

モノクロナール抗CD4

clone Q4120, purified from hybridoma cell culture

• 別名: Monoclonal Anti-CD4
• UNSPSCコード: 12352203
• NACRES: NA.44

特性

• 由来生物: mouse
• 結合体: FITC conjugate
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: Q4120, monoclonal
• フォーム: buffered aqueous solution
• 分子量: antigen 59 kDa
• 交差性: human
• テクニック: flow cytometry: 10 μL using 1 × 10⁶ cells
• アイソタイプ: IgG1
• UniProtアクセッション番号: P01730
• 輸送温度: wet ice
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... CD4(920)

詳細

詳細

Anti-CD4-FITC antibody, mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cell line NS-1 and splenocytes from Balb/c mice immunized with cluster of differentiation 4 (CD4)-transfected mouse T cell hybridoma, 3DT, followed by CD4⁺ human T cell CEM cells (leukemic lymphoblasts).

CD4, a glycoprotein, is predominantly expressed on the cell membrane of mature, thymus-derived (T) lymphocyte and also to some extent in the monocyte/macrophage lineage. It has a transmembrane domain, cytoplasmic region and an extracellular region of four tandem domain similar like members of Ig superfamily.

特異性

CD4を認識します。クロ-ン番号Q4120モノクロ-ナル抗体が認識するエピト-プは、アミノ酸1-183に位置しており、ホルマリン固定およびパラフィン包埋の影響をうけます。5th Workshop: code no. CD04.11

免疫原

CD4をトランスフェクトしたマウスT細胞ハイブリド-マ3DT、続いてCD4+ヒトT細胞由来CEM細胞

アプリケーション

FITC Conjugated Monoclonal Anti-Human CD4 has been used for:

• identification, quantification and monitoring of helper/inducer T cells in peripheral blood, biological fluids, lymphoid organs, and other tissues
• analysis of T cell mediated cytotoxicity
• characterization of subtypes of T cell leukemias and lymphomas
• studies of T cells in health and disease
• flow cytometry

生物化学的／生理学的作用

CD4⁺ T cells acts as a helper/inducer, in providing an activating signal to B cells. It induces T lymphocytes bearing the reciprocal CD8 marker to become cytotoxic/suppressor cells. It is also majorly associated with non-polymorphic region of MHC (major histocompatibility complex) antigen in interacting with targets bearing MHC class II molecules. Thus, it is called as an adhesion molecule. It also behaves as receptor for the GP 120 envelope glycoprotein of human immunodeficiency virus. The CD4 molecules is involved in the adhesion of T lymphocytes to target cells, thymic development, transmission of intracellular signals during T cell activation, and binding to polyclonal immunoglobulins.

Immunoregulatory T cell subset abnormalities in autoimmunity disorders, immunodeficiency diseases, graft versus host disease and following immunosuppressive therapy are often manifested as a change in CD4⁺/CD8⁺ ratio in peripheral blood T cells.

ターゲットの説明

CD4は、免疫グロブリン・ス-パ-ファミリ-に由来する単一鎖の膜貫通型糖タンパク質です。CD4は、ヘルパ-/インデュ-サ-T細胞サブセットをはじめ、大部分の胸腺髄質細胞、あるいはミクログリア細胞、樹状細胞やT細胞起源のある種の悪性腫瘍に発現しています。CD4は、MHCクラスII分子に結合し、p56^lckプロテインチロシンキナ-ゼと結合します。

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

調製ノート

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)