F1283

Fetal Bovine Serum

Fetal Bovine Serum, USA origin, Ultra Low IgG

• 別名: FBS, FCS, Fetal Calf Serum
• UNSPSCコード: 12352207
• NACRES: NA.71

特性

• 由来生物: bovine fetus
• 品質水準: 500
• 無菌性: sterile-filtered
• 製品種目: FBS Select
• 組成: Bovine IgG, <5 μg/mL ; Hemoglobin, ≤20 mg/dL
• 由来: USA origin
• 保管条件: avoid repeated freeze/thaw cycles; protect from light
• テクニック: cell culture | mammalian: suitable
• 不純物: ≤10 EU/mL endotoxin
• pH: 6.8-8.1
• オスモル濃度: 260-330 mOsm/kg H₂O
• 輸送温度: dry ice
• 保管温度: −20°C

詳細

詳細

Animal serum is commonly used to supplement basal media formulations for the optimal growth of many cell types in vitro.

Fetal Bovine Serum (FBS) is the most common serum used to supplement cell culture media due to its high nutritional content. Although it is relatively low in protein, FBS is effective in promoting and sustaining growth of vertebrate mammalian and insect cells.

IgG is the most common immunoglobulin present in FBS. Elevated levels of IgG may cause problems within certain recombinant protein or monoclonal antibody purification processes. For applications where IgG may interfere or cause issues, we have depleted IgG levels in this fetal bovine serum by sequential chromatography down to a level of < 5 ug/mL, while maintaining performance equivalent to regular FBS.

包装

Packaged in sterilized, graduated plastic bottles and sealed with a tamper indicator. Bottles are immediately frozen and stored at -10 to -40 C.

調製ノート

The fetal blood is aseptically collected and allowed to clot under controlled conditions. After centrifugation, the raw (unfiltered) serum is decanted from the clot, pooled and immediately frozen.

Frozen raw serum is thawed under controlled conditions and then processed through a series of membrane filters in descending pore size. FBS is filtered through three 0.1 μm filters before packaging.

アナリシスノート

Endotoxin and hemoglobin tested

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable