E3906

抗初期エンドソーム抗原1(C末端)抗体 ウサギ宿主抗体

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• 別名: 抗EEA1抗体, 抗エンドソーム関連タンパク質p162抗体, 抗ジンクフィンガーFYVEドメイン含有タンパク質2
• MDL番号: MFCD01633310
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 分子量: antigen ~160 kDa
• 化学種の反応性: mouse, rat, human
• 濃度: ~1 mg/mL
• テクニック: indirect immunofluorescence: 5-10 μg/mL using rat NRK cells; western blot (chemiluminescent): 0.1-0.2 μg/mL using whole extract of human HeLa cells; western blot (chemiluminescent): 0.2-0.4 μg/mL using whole extract of mouse NIH3T3 cells
• UniProtアクセッション番号: Q15075
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... EEA1(8411) ; mouse ... Eea1(216238) ; rat ... Eea1(314764)

詳細

詳細

Anti-Early Endosomal Antigen 1 (C-terminal), is produced in rabbit using a synthetic peptide corresponding to amino acid residues 1391-1410 of human EEA1, conjugated to keyhole limpet hemocyanin (KLH), as immunogen. Early Endosomal Antigen 1 (EEA1) is a 162 kDa autoantigen, which encodes for around 1400 amino acid proteins. EEA1 is a dimer, which comprises extensive coiled-coil regions. At its C-terminus, it contains a cysteine-rich zinc-finger-like domain named FYVE domain. The gene Early Endosome Antigen 1 (EEA1) encodes for around 1400 amino acid proteins

免疫原

ヒトEEA1の1391~1410残基に相当する合成ペプチドのKLH結合体。相当する配列はマウスと同一です。

アプリケーション

Anti-Early Endosomal Antigen 1 (C-terminal) antibody produced in rabbit has been used in:

• fluorescent staining
• confocal laser scanning microscopy
• immunoprecipitation
• indirect immunofluorescence microscopy
• fluorescence microscopy
• immunoblotting
• immunofluorescence.

生物化学的／生理学的作用

Anti- Early Endosomal Antigen 1 (EEA1) may be used as an early endosome marker. The binding of EEA1 to phosphatidylinositol 3-phosphate (PtdIns3P) and Rab5-GTP is essential for the localization and function of EEA1 in endocytic membrane fusion. It is associated with subacute systemic lupus erythematosus that specifically localized to early endosomes.

The gene Early Endosome Antigen 1 (EEA1) encodes for around 1400 amino acid proteins. It is a peripheral membrane protein associated with the cytoplasmic side of early endosomes. EEA1 controls vesicle fusion during endocytosis. In neurons, it is involved in recycling of synaptic vesicles and neurotransmitter receptors. Patients with neurological deficits develop EEA1 autoantibodies. Mutation in EEA1 gene leads to susceptibility to diabetes in the Japanese population.

物理的形状

0.01M PBS溶液(pH 7.4, 15mMアジ化ナトリウム含有)

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)