E4156

Anti-Early Endosomal Antigen 1 (N-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• 別名: 抗EEA1抗体, 抗エンドソーム関連タンパク質p162抗体, 抗ジンクフィンガーFYVEドメイン含有タンパク質2
• MDL番号: MFCD01633310
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 分子量: antigen ~160 kDa
• 化学種の反応性: mouse, human, rat
• 濃度: ~1 mg/mL
• テクニック: indirect immunofluorescence: 5-10 μg/mL using human HeLa and rat NRK cells; western blot (chemiluminescent): 0.4-0.8 μg/mL using whole extract of mouse NIH-3T3 cells
• UniProtアクセッション番号: Q15075
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... EEA1(8411) ; mouse ... Eea1(216238) ; rat ... Eea1(314764)

詳細

詳細

The gene Early Endosome Antigen 1 (EEA1) encodes for around 1400 amino acid proteins. It is a peripheral membrane protein associated with the cytoplasmic face of early endosomes. It is a 162 kDa autoantigen protein. EEA1 is a dimer, which comprises extensive coiled-coil regions. At its C-terminus, it contains a cysteine-rich zinc-finger-like domain named FYVE domain. This FYVE domain is conserved from yeast to man. FYVE domain is implicated in the specific localization of EEA1 to endosomes.

免疫原

ヒトEEA1のアミノ酸残基24~40に相当する合成ペプチド(C末端システイン付加)のKLH結合体。この配列はマウスと同一です。

アプリケーション

Anti-Early Endosomal Antigen 1 (N-terminal) antibody produced in rabbit has been used in immunoblotting and immunofluorescence.

生物化学的／生理学的作用

Early Endosomal Antigen 1 (EEA1) localization in endosomes is implicated in subacute systemic lupus erythematosus. Endosomal targeting of EEA1 also requires its binding to the active form of the small GTPase Rab5. The binding of EEA1 to phosphatidylinositol 3 phosphate (PtdInsP) and rabaptin-5 (Rab5)-GTP is essential for the localization and function of EEA1 in endocytic membrane fusion. Anti-EEA1 may be used as an early endosome marker.

物理的形状

0.01 M PBS溶液(pH 7.4, 15 mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)