おすすめの製品
形状
aqueous suspension
suspension
特徴
For purification of biotinylated macromolecules and complexes
濃度
50%
マトリックス
4% agarose (beaded)
マトリックス活性基
cyanogen bromide
保管温度
2-8°C
詳細
ExtrAvidin® is a unique de-glycosylated Avidin modified form of an affinity purified egg white avidin. The ExtrAvidin is conjugated to cyanogen bromide-activated agarose beads.
ExtrAvidin® is a tetrameric protein containing four biotin binding sites. The Avidin-Biotin high affinity interaction (Kd=10-15M) is considered one of the strongest non-covalent interactions known in nature. The use of Avidin-Biotin complex for affinity purification was described in the first half of 1970 and since then it was successfully utilized in numerous studies and biotechnological applications.1-3 This was achieved due to the ability to chemically coupe the small Biotin molecule with different binders, without disturbing its function or structure, thus allowing unique interaction with a variety of Avidin carriers including: protein or DNA molecules, Avidin protein bound to a solid surface matrix, reporter molecules, probes or carriers,. Among the known Avidin-Biotin interaction based applications are purification, enrichment, detection, amplification and other research medical and industrial processes. In addition, the ExtrAvidin® high specificity binding to Biotin, together with the low background staining, grant it significant advantage compared to the non-modified Avidin or Streptavidin produced by Streptomyces avidinii.
ExtrAvidin® is a tetrameric protein containing four biotin binding sites. The Avidin-Biotin high affinity interaction (Kd=10-15M) is considered one of the strongest non-covalent interactions known in nature. The use of Avidin-Biotin complex for affinity purification was described in the first half of 1970 and since then it was successfully utilized in numerous studies and biotechnological applications.1-3 This was achieved due to the ability to chemically coupe the small Biotin molecule with different binders, without disturbing its function or structure, thus allowing unique interaction with a variety of Avidin carriers including: protein or DNA molecules, Avidin protein bound to a solid surface matrix, reporter molecules, probes or carriers,. Among the known Avidin-Biotin interaction based applications are purification, enrichment, detection, amplification and other research medical and industrial processes. In addition, the ExtrAvidin® high specificity binding to Biotin, together with the low background staining, grant it significant advantage compared to the non-modified Avidin or Streptavidin produced by Streptomyces avidinii.
アプリケーション
ExtrAvidin®-Agarose provides high affinity with high specificity binding to Biotin (also known as vitamin B7) including biotinylated proteins and biotin-tagged fusion proteins. The product may be used in various immunological techniques, including Immunoprecipitation and Immunoaffinity purification.
注意
DO NOT FREEZE
物理的形状
ExtrAvidin®-Agarose is provided as suspension at a 1:1 ratio, in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.
法的情報
ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
This product is for research use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
保管分類コード
10 - Combustible liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
E2513-BULK:
E2513-VAR:
E2513-1ML:
E2513-5ML:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
この製品を見ている人はこちらもチェック
Samantha Ames et al.
Oncogene, 39(8), 1710-1723 (2019-11-15)
Tumor cells rely on glycolysis to meet their elevated demand for energy. Thereby they produce significant amounts of lactate and protons, which are exported via monocarboxylate transporters (MCTs), supporting the formation of an acidic microenvironment. The present study demonstrates that
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）