E1388

β-内皮細胞増殖因子 ヒト

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• 別名: ECGS, β-ECGF
• CAS番号: 105843-51-4
• MDL番号: MFCD00131012
• UNSPSCコード: 12352202
• NACRES: NA.77

特性

• product name: β-内皮細胞増殖因子 ヒト, β-ECGF, recombinant, expressed in E. coli, suitable for cell culture
• 由来生物: human
• リコンビナント: expressed in E. coli
• アッセイ: ≥97% (SDS-PAGE)
• 形状: lyophilized powder
• 有効性: 0.03-0.3 ng/mL ED₅₀/EC₅₀
• 品質: endotoxin tested
• 分子量: predicted mol wt ~17 kDa
• 包装: pkg of 25 μg
• 保管条件: avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)
• テクニック: cell culture | mammalian: suitable
• 不純物: ≤1.0 EU/μg protein Endotoxin level (LAL test)
• UniProtアクセッション番号: P49765
• 保管温度: −20°C
• 遺伝子情報: human ... VEGFB(7423)

詳細

詳細

The β-endothelial cell growth factor (b-ECGF) gene encodes an angiogenic factor, and is mapped to human chromosome 11q13.1. Recombinant, human b-endothelial cell growth factor (b-ECGF) is a 14 amino acid N-terminal extension of acidic fibroblast growth factor. It is expressed in Escherichia coli. The 154 amino acid recombinant protein, generated after removal of the initiation methionine, has a predicted molecular mass of about 17 kDa.

アプリケーション

β-Endothelial Cell Growth Factor human has been used:

• in cell death assay
• in endothelial cell culture
• in human umbilical vascular endothelial cell (HUVEC) culture

生物化学的／生理学的作用

β-Endothelial cell growth factor (β-ECGF) is a potent endothelial cell mitogen and its mitogenic activity is potentiated by heparin. β-ECGF stimulates the growth of quiescent HUVEC cells in culture. β-ECGF is associated with lipid and glucose metabolism. This growth factor induces neovascularization in disease conditions. Upregulation of β-ECGF is observed in diabetic retinopathy, and polycystic ovary syndrome. It is also associated with tumor proliferation and invasion. β-ECGF increases fatty acid transport proteins expression to improve endothelial mediated lipid transpor.

その他情報

N末端側に14アミノ酸の延長部分があるヒト酸性線維芽細胞成長因子(FGF)です。

物理的形状

凍結乾燥品(0.2μmフィルタ-で減菌濾過済み, 10 mM MOPS溶液, 50 mM硫酸ナトリウム, 0.2 mM DTT, 0.2 mM EDTA, pH 7.2, サイトカイン1 μgあたり50μgのBSAを含有)。

アナリシスノート

増殖促進活性は、静止期にあるNR6R-3T3線維芽細胞を用いて試験しました。

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, type N95 (US)