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リコンビナント
expressed in HEK 293 cells
アッセイ
95% (SDS-PAGE)
形状
lyophilized powder
テクニック
cell culture | mammalian: suitable
保管温度
−20°C
詳細
Nidogen-1 belongs to the nidogens family of glycoproteins. This family consists of nidogen-1 and nidogen-2, both of which are sulfated monomeric glycoproteins. Nidogen-1 is ubiquitously present in the synaptic and extra synaptic basal lamina. It is mapped to human chromosome 1q42.3. Nidogen-1 encodes a 139 kDa, three globular domains namely G1, G2 and G3 and has multiple epidermal growth factor repeats. Recombinant human nidogen-1 is expressed in human HEK 293 cells as a glycoprotein with a calculated molecular mass of 133.5 kDa (amino acids 29-1247). This protein is manufactured in human cells, with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports higher specific activity of the protein. The protein is produced with no artificial tags.
アプリケーション
This recombinant human nidogen-1 product can be used as an attachment factor for cells adherence.
生物化学的／生理学的作用
Nidogen-1 (NID-1) is thought to have an important role in the construction of basement membrane by linking between the networks of laminin and collagen IV. Genetic elimination of the nidogen-1 binding site on the mouse laminin γ1 chain leads to impaired deposition of basement membranes and hampers microvessel maturation. Nidogen 1 and 2 double knockout in mouse fibroblasts led to the loss of all major basal membrane components from the epidermal-collagen interface, and complete abolishment of hemidesmosomes. Supplementation by either recombinant nidogen-1 or -2 improved basal membrane formation. Mutations in NID1and laminin γ1 is implicated in pathogenesis of Dandy-Walker spectrum disorders.
保管分類コード
13 - Non Combustible Solids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
D8935-50UG-PW:
D8935-50UG:
D8935-VAR:
D8935-BULK:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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Mutations in Extracellular Matrix Genes NID1 and LAMC1 Cause Autosomal Dominant D andy-W alker Malformation and Occipital Cephaloceles
Darbro BW, et al.
Human Mutation, 34(8), 1075-1079 (2013)
Structural elucidation of full-length nidogen and the laminin-nidogen complex in solution
Patel TR, et al.
Matrix Biology, 33, 60-67 (2014)
Basement Membrane Type IV Collagen and Laminin: An Overview of Their Biology and Value as Fibrosis Biomarkers of Liver Disease
Mak KM, et al.
The Anatomical Record, 300(8), 1371-1390 (2017)
Skin basement membrane: the foundation of epidermal integrity?BM functions and diverse roles of bridging molecules nidogen and perlecan
Breitkreutz D, et al.
BioMed Research International, 2013 (2013)
Analysis of nidogen-1/laminin gamma1 interaction by cross-linking, mass spectrometry, and computational modeling reveals multiple binding modes
Lossl P, et al.
Testing, 9(11), e112886-e112886 (2014)
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