CZFA2136

CompoZr^® ZFA Targeting Human SOX2

• UNSPSCコード: 12352200
• NACRES: NA.51

特性

• 製品種目: CompoZr^®
• 品質水準: 200
• 輸送温度: dry ice
• 保管温度: −70°C

詳細

詳細

Zinc finger nucleases (ZFNs) are a class of engineered DNA-binding proteins that facilitate targeted gene editing. Now, Sigma-Aldrich provides ZF activators, repressors and epigenetic modifiers for gene expression studies. ZFAs, or similarly called ZF−TFs (zinc finger transcription factors) incorporate a transcriptional activation domain in place of the nuclease domain found in ZFNs and specifically activate coding and non-coding genes. The increase in specific gene expression is dependent on the target binding site and the effector domain selected. Native gene activation is through ZFAs binding to promoters and recruiting the transcriptional machinery. This approach for activation also has the benefit of expressing endogenous isoforms at the proper ratio.
*Please note all ZFA plasmids are made standard with the p65 activation domain. The end-user will have to swap out the p65 domain and clone in the other activation, epigenetic or repressor domains as needed.

特徴および利点

CompoZr ZFAs allows researchers to over-express endogenous genes with the advantage of having all splice variants in the appropriate ratios.

• Validated ZFAs targeting SOX2 (human).
• ZFA is a powerful, novel tool to modulate gene expression in human cells with low potential for off-targets.

構成

• Validated ZFA plasmids with the p65 activation domain to upregulate human SOX2.

調製ノート

Transcription Factor p65, or the RelA NF-kappaB (nuclear factor kappaB) subunit, contains a C-terminal transcriptional activation domain.Additional activation domains are included in the kit, which include plasmids with the 2xp65, VP16 and VP64 activation domains.2xp65 consists of two repeating units of the p65 activation domain.VP16 is a trans-acting domain from the herpes simplex virus that induces early gene transcription.VP64 is a transactivating domain made up of a tetrameric repeat of VP16 minimal activation domain.

その他情報

If you are interested in the CompoZr ZFN technology for permanent editing of your gene of interest, please inquire about our Custom ZFN Service offering at: ZFNOps@sial.com

法的情報

CompoZr is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable