CMC0015

BL21(DE3) pLysE Chemically Competent Cells

Escherichia coli, rod shaped

• UNSPSCコード: 41106202
• NACRES: NA.85

特性

• product name: BL21(DE3) pLysE Chemically Competent Cells, for protein expression
• 由来生物: Escherichia coli
• グレード: for molecular biology
• 成長モード: adherent or suspension
• 形態: rod shaped
• テクニック: microbiological culture: suitable
• 細胞形質転換: competent cell type: chemically competent; transformation efficiency: ≥1 x 10⁷ cfu/μg
• 輸送温度: dry ice
• 保管温度: −70°C

詳細

詳細

The BL21(DE3) pLysE Chemically Competent Cells produce T7 lysozyme to repress low-level transcription from the T7 promoter prior to IPTG induction, helping to stabilize inserts coding for deleterious products.
These cells have a higher level of repression by T7 lysozyme, offering the greatest control over expression of inserts.

Genotype

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

特徴および利点

• a transformation efficiency of >1 × 10⁷ cfu/μg.
• economical prices
• offering superior value for everyday protein expression work.

構成

• BL21(DE3) pLysE chemically competent cells
• pUC 19 transformation control DNA
• recovery medium for expression

安全性情報

• 保管分類コード: 10 - Combustible liquids