DI TNC1

98102214, rat brain, Neuronal (astrocyte)

• UNSPSCコード: 41106514

特性

• product name: DI TNC1, 98102214
• 由来生物: rat brain
• 成長モード: Adherent
• 核型: Not specified
• 形態: Neuronal (astrocyte)
• 製品: Alpha-2 macroglobulin
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Rat Astrocyte transfected

細胞株の説明

Established by transfecting cultures of primary astrocytes from diencephalon tissue of a 1 day old sprague-dawley rat with a DNA construct containing the oncogenic early region of SV40. Transcriptional control was affected using the human GFAP promoter (pGFA-SV-Tt) and the murine phosphoglycerate kinase promoter (pPGK-neo). Cloning was achieved using G418. The cells are phenotypically similar to type 1 astrocytes, including immunoreactivity to glial fibrillary acid protein (GFAP) and a β-alanine inhibitable high-affinity uptake mechanism for gamma amino butyric acid (GABA). α-2-macroglobulin production is similar to that found in primary astrocytes, but transferrin production is reduced. The cell line has been shown not to produce proenkephalin A, galactocerebroside or to express the 04 or A2B5 epitopes characteristic of type 2 astrocytes. Immunostaining has shown the SV40 T antigen to be present in over 90% of cells.

アプリケーション

Studies of the development and biochemistry of the central neurosystem

培地

DMEM + 4mM Glutamine + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

継代と培養方法

Split sub-confluent cultures (70-80%) at 1:2 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Subculture before flasks become confluent or cells will detach in sheets reducing effectiveness of trypsin.

その他情報

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