Clone 15 HL-60

98052918, human unknown/unspecified, Lymphoblastoid

• UNSPSCコード: 41106514
• 由来生物: human unknown/unspecified
• 成長モード: Suspension
• 核型: Not specified
• 形態: Lymphoblastoid
• 製品: Not specified
• 受容体: IL-5
• 関連疾患: cancer

特性

• 製品名: Clone 15 HL-60, 98052918
• 由来生物: human unknown/unspecified
• 成長モード: Suspension
• 核型: Not specified
• 形態: Lymphoblastoid
• 製品: Not specified
• 受容体: IL-5
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human caucasian promyelocytic leukaemia

細胞株の説明

The clone 15 HL-60 has been derived from HL-60 cells (Sigma Catalogue number. 98070106) that had been cultured at an elevated pH of 7.6 - 7.8 for 2 months. Clone 15 HL-60 was picked from soft agar being selected for the ability to differentiate to eosinophils after treatment with butyric acid. Although 80% of the cells will exhibit this differentiation pattern initially, reversion will occur during culture leading to a higher percentage reverting to neutrophilic differentiation. Expression of IL-5 receptors and eosinophil granule proteins has been found (eosinophil major basic protein (MBP), eosinophil peroxidase (EPO), eosinophilotactions protein (ECP) and eosinophil derived neurotoxin (EDN). In addition, chemotaxis to eosinophil specific eosinophilotactius has been described.

アプリケーション

Leukemia studies, oncogene expression, differentiation assays

培地

RPMI 1640 + 2mM グルタミン + 10％ ウシ胎児血清（FBS）

継代と培養方法

Maintain cultures between 3-9 x 100,000 cells per ml; 5% CO₂; 37°C. Immediately after resuscitation use medium with 20% foetal bovine serum (FBS) to aid establishment of growing culture. Reduce FBS concentration to 10% once cells are well established in

その他情報

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