293-PERV-PK-CIRCE

97051411, human kidney, Not specified

• UNSPSCコード: 41106514

特性

• 製品名: 293-PERV-PK-CIRCE, 97051411
• 由来生物: human kidney
• 包装: tube of 5 μg 97051411-DNA-5UG; pkg of vial of cells 97051411-1VL
• 成長モード: Adherent aggregates
• 核型: Not specified
• 形態: Not specified
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human embryo kidney, infected with endogenous retrovirus of pigs

細胞株の説明

293-PERV-PK-CIRCE has been established by infecting the human kidney 293 cell line (Sigma Catalogue number. 85120602) with retrovirus produced by the pig kidney cell line PK-15. The endogenous, C-type retrovirus particles produced by PK-15 have been shown to be capable of infection, replication and serial transfer. 293-PERV-PK-CIRCE is useful to examine the host range of the pig retrovirus to assess the risks associated with porcine xenotransplantation. The cell line has tested negative for Hog Cholera virus. Handle under ACDP Category 2 conditions.

アプリケーション

Pig retrovirus studies, infectivity assays

培地

DMEM + 2mM Glutamine + 10% HI-FBS

継代と培養方法

Split sub-confluent cultures (70-80%) 1:6 cells can be dislodged by mechanical agitation 5% CO₂ 3

その他情報

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