JVM-2

96090515, human unknown/unspecified, Lymphoblastoid

• UNSPSCコード: 41106514

特性

• product name: JVM-2, 96090515
• 由来生物: human unknown/unspecified
• 成長モード: Suspension
• 核型: 46,XX,-8,+t(3;8)(p12-13;q12),t(11;14)(q13;q32)
• 形態: Lymphoblastoid
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human Caucasian B-prolymphocytic leukaemia, EBV-transformed

細胞株の説明

JVM-2 has been derived from EBV and TPA treated mononuclear cells from a 63 year old female patient with B-prolymphocytic leukaemia. Thirty-three percent of cells were shown to be positive for surface lambda light chain, 37% for IgA, 29% for IgG and 3% for IgM and IgD. In addition, 50% were found to express cytoplasmic IgM and lambda light chain. Expression of the following leukocyte differentiation antigens was found: CD9, CD19, CD20, CD23, CD24, MA6, CD37, CD38, FMC1, FMC7, MHC Class I, FMC16, MHC Class II DR/DP/DQ. The immunoglobulin heavy-and light-chain genes showed the same pattern of rearrangement as in the original prolymphocytes. The cells are lymphoblastoid with large floating aggregates present. The cell line has been eradicated from mycoplasma at ECACC.

アプリケーション

Ig expression of neoplastic cells

DNAプロファイル

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11,13
D16S539: 12,13
D5S818: 11,12
D7S820: 10,11
THO1: 6,9
TPOX: 8,11
vWA: 17

培地

RPMI 1640 +2mM Glutamine + 10% Foetal Bovine Serum (FBS).

継代と培養方法

Maintain cultures between 3-9 x 100,000/ml; 5% CO₂; 37°C.

その他情報

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.