My-La CD4+

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 95051032, human lymph, Lymphoblastoid

• 別名: My-La CD4+ Cells
• UNSPSCコード: 41106514

特性

• 由来生物: human lymph
• 包装: tube of 5 μg 95051032-DNA-5UG; pkg of vial of cells 95051032-1VL
• 成長モード: Suspension
• 核型: Not specified
• 形態: Lymphoblastoid
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human Caucasian CD4+ T Lymphocyte, mycosis fungoides

細胞株の説明

Two different continuous cell lines were established from a plaque biopsy of an 82-year old male with mycosis fungoides stage II by inclusion of IL-2 and IL-4 in the culture medium: My-La, CD4+, (referred to in the literature as My-La, marker chromosome) and My-La, CD8+ (referred to in the literature as My-La 46,XY-18+i(18q) (Sigma Catalogue number. 95051033). My-La CD4+ is strictly dependent on exogenous IL-2 and IL-4 in the culture medium whereas My-La CD8+ on IL-2 only. Both show characteristic chromosome markers and are double homozygous for HLA-A1.B8. My-La CD8+ expresses β-13 of the T cell receptor and phenotypically weak staining was observed for CD2, CD3, CD8, CD25, CD71 and TCR-1 α / ß. My-La CD4+ expresses β-18 of the T cell receptor plus staining for CD2, CD3, CD4,CD25, CD71, HLA-DR and TCR-1 α/ ß. There has been no evidence of HTLV-I infection in the cell lines and serum from the patient did not demonstrate antibodies against HTLV-I and HTLV-II. Both cell lines show a low viability after resuscitation from frozen and a long lag time phase until exponential growth is reached.

アプリケーション

Study of antigen-independent proliferation of T- cell lyphoma from mycosis fungoides patient.

DNAプロファイル

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,12
D16S539: 8,11
D5S818: 9,12
D7S820: 10,11
THO1: 6
TPOX: 8,12
vWA: 17

培地

RPMI 1640 (R0883) + 2mM L-Glutamine (G7513) + 10 U/ml IL-2 (H7041) + 10 U/ml IL-4 (H7291) + 10% Human AB Serum (H4522)

継代と培養方法

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO₂; 37°C. Split only 1:2 as maximum dilution: freeze in 45% growth medium, 45% human AB serum plus 10% DMSO, low viability on resuscitation. Use of human serum produces high levels of debris in cultures. Population doubling time 20-24 hours.

その他情報

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.