FGC4

94101904, rat skin, Epithelial

• UNSPSCコード: 41106514

特性

• product name: FGC4, 94101904
• 由来生物: rat skin
• 成長モード: Adherent
• 核型: Modal no. 52
• 形態: Epithelial
• 製品: Albumin and ß-fibrogen
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Rat hepatoma [alb-gpt plasmid]

細胞株の説明

A subclone derived from Fao which is deficient in HGPRT (resistant to 5μg/ml 6-thioguanine and also resistant to 3mM ouabain). FGC4 contains the alb-gpt plasmid which is the bacterial gpt gene under control of the rat albumin promoter (400 bp). Produces albumin and ß-fibrinogen.GMO1

アプリケーション

Gene expression studies.

培地

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

継代と培養方法

Split semi-confluent cultures (70%) 1:100 i.e. seeding at 2x1000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Media change three times a week.

その他情報

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