DLD-1

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 90102540, human colon, Epithelial

• UNSPSCコード: 41106514

特性

• 由来生物: human colon
• 包装: tube of 5 μg 90102540-DNA-5UG; pkg of vial of cells 90102540-1VL
• 成長モード: Adherent
• 核型: 2n = 46, pseudodiploid
• 形態: Epithelial
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human colon adenocarcinoma

細胞株の説明

Derived from human colorectal adenocarcinoma. The cells have been used in the study of polar solvents on cell characteristics.The four cell lines: DLD-1 (90102540), HCT-15 (91030712), HCT-8 (90032006) and HRT-18 (86040306) have been shown to have a common genetic origin.

アプリケーション

DLD-1 cell line has been used to express shRNA oligos directed against coronin 2A (CRN5) and to study the role of CRN5 in tumor cell migration. It has also been used to determine the efficiency of antibody-coupled short interfering RNA (siRNA) for in vivo mRNA knockdown.

DNAプロファイル

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,11
D16S539: 12,13
D5S818: 13
D7S820: 10,12
THO1: 7,9.3
TPOX: 8,11
vWA: 18,19

培地

RPMI 1640 + 2mM グルタミン + 10％ ウシ胎児血清（FBS）

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3 x 10,000 cells/cm² using 0.25% trypsin, 0.02% EDTA.

その他情報

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