NCTC 4093

90102530, mouse embryo, Fibroblast

• UNSPSCコード: 41106514
• 由来生物: mouse embryo
• 成長モード: Adherent
• 核型: Modal no. 71-73; hypotetraploid
• 形態: Fibroblast
• 製品: Not specified
• 受容体: Not specified

特性

• 製品名: NCTC 4093, 90102530
• 由来生物: mouse embryo
• 成長モード: Adherent
• 核型: Modal no. 71-73; hypotetraploid
• 形態: Fibroblast
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mouse C57B1/KaLw embryo

細胞株の説明

Derived by cloning from a parent line produced from normal mouse embryo tissue (C57B1/KaLw). The cells have spontaneously transformed in vitro. The cells are negative for polyoma virus infection but are susceptible to the virus.

アプリケーション

Transformation and virus studies: Polyoma virus

培地

NCTC 135 100% + 2mM Glutamine.

継代と培養方法

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using mechanical methods to dislodge the cells (e.g. scraping); 5% CO₂; 37°C.

その他情報

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