H4II

89042702, rat skin, Epithelial

• UNSPSCコード: 41106514

特性

• product name: H4II, 89042702
• 由来生物: rat skin
• 成長モード: Adherent
• 核型: 52 chromosomes
• 形態: Epithelial
• 製品: Albumin, transferrin, prothrombin
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Rat skin Hepatocellular carcinoma

細胞株の説明

From H-35 Reuber hepatoma induced by chemical carcinogenesis in an AxC rat . Tyrosine aminotransferase positive. Synthesises several serum proteins including albumin, transferrin and prothrombin. (Same as H4-11-E-C3 (Sigma Catalogue number. 85061112)).

アプリケーション

Gene expression analysis.

培地

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

継代と培養方法

Split semi-confluent [60%-70%] cultures 1:100 i.e. seeding at 2x100 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Saturation density is reached at 15x1000,000 cells/10cm² dish.

その他情報

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