L929/A

14112101, mouse adipose tissue, Fibroblast

• UNSPSCコード: 41106514

特性

• product name: L929/A, 14112101, from mouse adipose tissue
• 由来生物: mouse adipose tissue
• 詳細: Mouse C3H/An, connective tissue, Adriamycin,Doxorubicin, drug resistant
• 成長モード: Adherent
• 形態: Fibroblast
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の説明

This Adriamycin-resistant cell line has been developed by exposure of the parent L929 murine fibroblast cell line (ECACC catalogue number 85011425) to increasing concentrations of doxorubicin in-vitro. L929/A cells can be used in the development of novel anti-cancer treatments. Resistance can be circumvented by modulating agents such as verapamil and quinine. The parent cell line L929 was derived from normal subcutaneous areolar adipose tissue.

培地

RPMI 1640 + 10% Fetal Bovine Serum (FBS) + 2mM L-Glutamine + 0.086 uM Doxorubicin hydrochloride.

継代と培養方法

Trypsinize with 0.05% Trypsin/EDTA and inoculate new cultures vessels at 1-2 x 10⁴ cells/cm². Cultures incubated in a humidified 5% CO₂/95% air incubator at 37^oC.

その他情報

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Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.