HL60 Ast.4

88120802, human blood, Promyeloid, blast/promyelocyte

• UNSPSCコード: 41106514

特性

• 製品名: HL60 Ast.4, 88120802
• 由来生物: human blood
• 包装: tube of 5 μg 88120802-DNA-5UG; pkg of vial of cells 88120802-1VL
• 成長モード: Suspension
• 核型: 2n = 48, hyperdiploid
• 形態: Promyeloid, blast/promyelocyte
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human Caucasian promyelocytic leukaemia

細胞株の説明

Variant of the promyelocytic cell line HL60 isolated after treating cells with α-particle radiation and then cloned in medium containing 1.25% DMSO. Treatment with 50nM TPA results in only very limited differentiation towards basophilic morphology (Giemsa stain). Differentiation towards monocyte lineage has not been observed.

アプリケーション

Differentiation studies

培地

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1.25% Dimethyl Sulphoxide (DMSO).

継代と培養方法

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Do not allow the culture medium to become acidic.

その他情報

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