A375 Cell Line human

88113005, human skin, Epithelial

• 別名: A-375 Cells, A375-MEL Cells
• UNSPSCコード: 41106514

特性

• 製品名: A375 Cell Line human, 88113005
• 由来生物: human skin
• 詳細: Human malignant melanoma
• 成長モード: Adherent
• 核型: Modal no. = 62 (hypotriploid) 9 marker chromosomes. Normal N2, N6 and N22 present.
• 形態: Epithelial
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human malignant melanoma

細胞株の説明

Derived from a 54 year old female with malignant melanoma. The cell line produces rapidly growing amelanotic melanomas in anti-thymocyte serum treated NIH Swiss mice.

アプリケーション

発がん性の研究

A375 cell line has been used to obtain paracrine factors for prolonged culture of mesenchymal stromal cells (MSCs). It has also been used to study oncolytic activity of the peptide LTX-315.

培地

DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

その他情報

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