H9C2 (2-1) Cell Line from rat

88092904, rat cardiovascular , Myoblast

• 別名: H9c2 Cells
• UNSPSCコード: 41106514

特性

• product name: H9C2 (2-1) Cell Line from rat, 88092904, BDIX heart myoblast
• 由来生物: rat cardiovascular
• 成長モード: Adherent
• 核型: Not specified
• 形態: Myoblast
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Rat BDIX heart myoblast

細胞株の説明

Skeletal muscle properties. Myotubes formed at confluency respond to acetylcholine. Differentiation improved by reducing serum concentration to 1%.

アプリケーション

Differentiation studies

培地

DMEM + 2mM グルタミン + 10％ ウシ胎児血清（FBS）

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Important to passage at sub-confluence, reclone periodically to keep differentiated characteristics. Cells will fuse to form myotubes if allowed to reach confluency.

その他情報

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