COS-1 Cell Line

88031701, African green monkey kidney, Fibroblast

• UNSPSCコード: 41106514

特性

• 製品名: COS-1 Cell Line, 88031701, from African green monkey kidney, SV40 transformed, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)
• 由来生物: African green monkey kidney
• 成長モード: Adherent
• 核型: Not specified
• 形態: Fibroblast
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Monkey African green kidney, SV40 transformed

細胞株の説明

Derived from CV-1 simian cells transformed by origin-defective mutant of SV40 which codes for wildtype T Ag. Possible host for propagation of pure populations of recombinant SV40 virus. The cells should be handled under laboratory containment level 2.

アプリケーション

COS-1 cell line has been used to test the toxicity of various concentrations of iron oxide nanoparticles (for use in medical diagnostics). It has also been used to study the interaction of nanoparticles with their cellular targets.

Virus replication studies: SV40

培地

DMEM (D6546) + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442).

継代と培養方法

Split semi-confluent cultures 1:3 to 1:6, i.e., seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. In order to prevent syncitia formation, do not allow cells become confluent.

その他情報

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