Grunt Fin (GF)

88010601

• UNSPSCコード: 41106514

特性

• 由来生物: fish fin
• 成長モード: Adherent
• 核型: Modal no. 47
• 形態: Fibroblast-like
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Fish Grunt fin tissue blue striped

細胞株の説明

Derived from fin tissue of the adult salt water blue striped Grunt. Does not support the replication of influenza A, herpes simplex, adeno, polio, dengue and eastern equine encephalitis viruses.

培地

EMEM (HBSS) (Sigma cat no. M5775) + 10mM Hepes (pH 7.3) + 2mM Glutamine + 0.23% (w/v) NaHCO₃ + 20% FBS (Heat Inactivated)+ 58mM NaCl

継代と培養方法

Split sub-confluent cultures (70-80%) 1:2 to 1:4 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 20°C. After resuscitation split cells 1:2 to 1:4 and culture for at least one week with 1-2 media changes before shipping. Plating efficiency can be very low a

その他情報

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