KG-1

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 86111306, human bone marrow, Myeloblast-like

• 別名: KG1 Cells
• UNSPSCコード: 41106514

特性

• 由来生物: human bone marrow
• 包装: tube of 5 μg 86111306-DNA-5UG; pkg of vial of cells 86111306-1VL
• 成長モード: Suspension
• 核型: 2n = 46, diploid
• 形態: Myeloblast-like
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human Caucasian bone marrow myelogenous leukaemia

細胞株の説明

Derived from the bone marrow aspirate of a 59 year old male Caucasian with erythroleukemia that became acute myelogenous leukaemia. The cells form colonies in soft-agar in the presence of colony stimulating factor (CSF). KG-1 cells resemble acute myelogenous leukaemia showing considerable pleomorphism with a predominance of myeloblasts and promyelocytes. A small percentage of the cells are mature granulocytes with the occasional presence of macrophages and eosinophils. They do not express surface Ig or EBV antigens and lack specific markers for lymphocytic cells.

アプリケーション

KG-1 has been used to extract total RNA and use them as templates to determine the 5′ end of promyelocytic leukemia zinc finger (PLZF) transcripts.

DNAプロファイル

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 7
D13S317: 11,12
D16S539: 10,11
D5S818: 13
D7S820: 8,10
THO1: 7,8
TPOX: 7,9
vWA: 14,19

培地

IDMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

継代と培養方法

3～9×100,000細胞/mL、5％ CO₂、37℃の状態を維持して培養してください。

その他情報

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