3T3 clone A31 Cell Line from mouse

86110401, mouse embryo, Fibroblast

• 別名: 3T3-A31 Cells, BALB/3T3 clone A31 Cells, BALB/c 3T3 clone A31 Cells
• UNSPSCコード: 41106514

特性

• 製品名: 3T3 clone A31 Cell Line from mouse, 86110401
• 由来生物: mouse embryo
• 詳細: Mouse BALB/c embryo
• 成長モード: Adherent
• 核型: 2n = 40, hypotetraploid
• 形態: Fibroblast
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mouse BALB/c embryo

細胞株の説明

Cells have now lost their contact inhibition.

アプリケーション

3T3 clone A31 cell line from mouse has been used:

• to study in vitro cytotoxicity and genotoxicity of retinyl palmitate–loaded poly(lactide-co-glycolide) nanoparticles for the topical treatment of skin diseases
• for the characterization of the phototoxicity of Vemurafenib, which is a B-Raf serine/threonine kinase inhibitor
• for the phototoxicity assessment of systemically applied compounds and drug candidates

Tumourigenicity and virus studies: SV40 and MSV

培地

DMEM + 2mM Glutamine + 5% New Born Calf Serum (NBCS) + 5% Foetal Bovine Serum (FBS).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 5x1,000 to 2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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