A549 Cell Line human

86012804, human lung (carcinoma), Epithelial

• 別名: A-549 Cells, A549 Cells, NCI-A549 Cells
• UNSPSCコード: 41106514

特性

• product name: A549 Cell Line human, 86012804, from human lung(carcinoma)
• 由来生物: human lung (carcinoma)
• 成長モード: Adherent
• 核型: Hypotriploid
• 形態: Epithelial
• 製品: Lecithin, High amounts of de-saturated fatty acids.
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human Caucasian lung carcinoma

細胞株の説明

Derived from a 58 year old Caucasian male. The cells can synthesise lecithin utilising the cytidine diphosphocholine pathway. Occasional cells may also contain inclusion bodies although they are not known to carry any human pathogen.

アプリケーション

Enzymatic studies

Human A549 cell line has been used:

• to study the ability of 2-(2,4-dihydroxyphenyl)thieno-1,3-thiazin-4-one (BChTT) to inhibit cancer cell proliferation and identify mechanisms involved in its actions
• to study the effects of insulin and insulin-like growth factor 1 (IGF1) on proliferation and apoptosis in A549 cells
• to culture A549 cells in different media over a long term and identify conditions for promoting alveolar type II differentiation characteristics in A549 cells

DNAプロファイル

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 11
D7S820: 8,11
THO1: 8,9.3
TPOX: 8,11
vWA: 14

培地

Ham′s F12K + 2mM Glutamine + 10% Foetal Bovine Serum FBS / FCS.

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% Trypsin or tTrypsin/EDTA; 5% CO₂; 37°C.

その他情報

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