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MA104

85102918, African green monkey kidney, Epithelial

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About This Item

UNSPSCコード:
41106514
eCl@ss:
32190102

product name

MA104, from African green monkey kidney, 85102918, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)

由来生物

African green monkey kidney

成長モード

Adherent

核型

Not specified

形態

Epithelial

受容体

Not specified

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細胞株の由来

Monkey African Green kidney

細胞株の説明

Established from an African green monkey foetal kidney. The cells are highly susceptible to Simian rotavirus SA11.

Please note: The species of origin of the MA104 cell line has been shown to be different from that claimed by the originators.

Whitaker & Hayward (1985) found the cell line to originate from an African green monkey and not a Rhesus macaque as was originally claimed. Whitaker AM and Hayward CJ (1985). The characterization of three monkey kidney cell lines. Develop. Biol. Standard. Vol 60: 125-131. PMID: 4043530. Abstract: Three monkey kidney cell lines, Vero, GL-V3 and MA-104 were subjected to karyological analysis to determine their chromosomal stability and to confirm their species of origin. Although the lines were shown to be relatively stable throughout all of the passage levels that were tested, the species of origin of one of them was found to be different from that claimed by the originators. This finding was supported by data from isoenzyme studies.

培地

EMEM (EBSS) (M2279) + 2 mM L-Glutamine (G7513) + 1% NEAA (M7145) + 10% FBS / FCS (F2442).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e., seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C.

その他情報

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