AFRC MAC 77

85102262, Reactivity: Anti-Nitrate Reductase

• UNSPSCコード: 41106514

特性

• 由来生物: Rat x Rat Hybridoma
• ImmunoDon: F344 rat spleen
• 骨髄腫: Y3Ag1.2.3
• 反応性: Anti-Nitrate Reductase
• テクニック: cell culture | mammalian: suitable
• アイソタイプ: IgG2b
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Rat Y3Ag1.2.3 x Rat F344 spleen

細胞株の説明

The antibody specific for NADH nitrate reductase and inhibits the reduction of nitrate to nitrite using NADH as electron donor. It also inhibits the reduction of nitrate using flavin nucleotides (FMNH2 or FADH2) and methyl viologen. The antibody is directed against NADH-NR of mono and dicotyledons.

免疫原

Nitrate reductase purified from the leaves of 6 to 8 week old spinach plants - Spinacea oleracea l

培地

DMEM + 2mM グルタミン + 10％ ウシ胎児血清（FBS）

継代と培養方法

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. These cells require mouse macrophage feeder layers upon resuscitation

その他情報

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