A431 Cell Line human

85090402, human skin, Epithelial

• 別名: A-431 Cells, A431/P Cells
• UNSPSCコード: 41106514

特性

• product name: A431 Cell Line human, squamous carcinoma, 85090402
• 由来生物: human skin
• 成長モード: Adherent
• 核型: Hypertriploid
• 形態: Epithelial
• 製品: Not specified
• 受容体: Epidermal growth factor (EGF)
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human squamous carcinoma

細胞株の説明

Derived from an epidermal carcinoma of the vulva taken from an 85 yr old female. The cells carry large numbers of EGF binding sites and is an indicator cell for anti-TGF binding.

アプリケーション

A431 Cell Line human has been used:

• to demonstrate that p38 mitogen-activated protein kinase enhances epidermal growth factor-induced apoptosis in A431 cells
• to study melanoma-specific CD44 (a tumor marker and transmembrane receptor) alternative splicing pattern in A431 cells
• to study the anti-cancer activity of antioxidant-rich Cameroonian medicinal plants

Cell cycle studies

培地

EMEM（EBSS） + 2 mMグルタミン + 1％非必須アミノ酸（NEAA） + 10％ウシ胎児血清（FBS）

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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