KHOS/NP

84102903, human bone, Epithelial

• UNSPSCコード: 41106514

特性

• product name: KHOS/NP, 84102903
• 由来生物: human bone
• 成長モード: Adherent
• 核型: Not specified
• 形態: Epithelial
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human Caucasian osteosarcoma

細胞株の説明

Isolated from transformed foci of human osteogenic sarcoma (HOS) cells which were induced by Kirsten murine sarcoma virus. They contain MSV genome but do not produce infectious virus. The transformed cells exhibit a high saturation density, a high plating efficiency in soft agar and produce tumours in nude mice.

アプリケーション

Assay of type-C leukaemia viruses.

DNAプロファイル

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 8,11
vWA: 18

培地

EMEM（EBSS） + 2 mMグルタミン + 1％非必須アミノ酸（NEAA） + 10％ウシ胎児血清（FBS）

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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