W6/1

13082201, from Mouse x Mouse Hybridoma

• UNSPSCコード: 41106514

特性

• 由来生物: Mouse x Mouse Hybridoma
• ImmunoDon: BALB/c mouse spleen
• 骨髄腫: P3-NS1/1-Ag4.1
• 反応性: Human ′A′ erythrocytes
• テクニック: cell culture | mammalian: suitable
• アイソタイプ: IgM
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の説明

The W6/1 hybridoma cell line secretes monoclonal IgM antibodies reactive with a polymorphic determinant of human erythrocytes which correlated with blood group A.

免疫原

Human tonsil membrane

アプリケーション

Human erythrocyte marker.

培地

RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)

継代と培養方法

Seed at 5 x 10⁴ cells/ml. Subculture when cells reach 5 x 10⁵ -1 x 10⁶ cells/ml (usually after 3-4 days), 5% CO₂, 37°C. Start in 20% FBS when recovering from frozen.

その他情報

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Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.