JU77 cell line human

10092309, human lung, epithelioid: spindly with few vacuoles. Cobblestone morphology at confluency.

• UNSPSCコード: 41106514

特性

• 製品名: JU77 cell line human, 10092309
• 由来生物: human lung
• 詳細: Malignant mesothelioma cells from human lung
• 包装: tube of 5 μg 10092309-DNA-5UG; pkg of vial of cells 10092309-1VL
• 成長モード: Adherent
• 核型: Not specified
• 形態: Epithelial-like: cells are spindle-shaped with few vacuoles. Upon confluence develop an epithelioid "cobblestone-like mat"
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human malignant mesothelioma

細胞株の説明

This cell line was derived from the pleural fluid of a patient with malignant mesothelioma. The patient had known exposure to crocidolite asbestos. JU77 is not tumourigenic in nude mice. Cells express cytokeratin and epithelial membrane antigen (EMA) but not mucin. Cells are spindle shaped with few vacuoles. Cell Bank Australia reference number: CBA0140

アプリケーション

Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.

培地

RPMI 1640 (with 2mM L-Glutamine+ 25mM HEPES) + 5% FCS

継代と培養方法

Optimal split ratio 1:8 (seeding density 0.8 x104 cells/cm²).Cells incubated at 37°C with 5% CO₂.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.

その他情報

HPA Culture Collections由来でSIGMAによって供給される培養物は研究の目的のみに使用できます。商業目的での細胞株の使用に関する質問は、その細胞株のデポジターに照会されます。一部の細胞株には、細胞株の供給の前に受給予定者によって物質移動契約書への記入を完了させるという要求など、さらなる特別な公開条件があります。詳しくはTerms & Conditions of Supplyをご覧ください。

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Depositor and originator: Richard Lake - University of Western Australia, Australia