CAD cell line from mouse

8100805, mouse nerve, Neuronal

• 別名: CAD Cell Line
• UNSPSCコード: 41106514

特性

• 製品名: CAD cell line from mouse, 08100805
• 由来生物: mouse nerve
• 詳細: Mouse (B6/D2 F1 hybrid) catecholaminergic neuronal tumor
• 成長モード: Adherent
• 核型: Not specified
• 形態: Neuronal
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mouse (B6/D2 F1 hybrid) catecholaminergic neuronal tumour

細胞株の説明

CAD cells (Cath.-a-differentiated) are a variant of a CNS catecholaminergic cell line established from a brain tumour in a transgenic mouse carrying wild-type SV40 T antigen under transcriptional control of rat tyrosine hydroxylase promoter. CAD has lost the immortalising oncogene and expresses neurone-specific proteins and synaptic vesicle proteins. CAD will differentiate to form neuronal processes in serum free conditions.

アプリケーション

Study of neuronal differentiation

培地

DMEM:HAMS F12 (1:1) + 2mM Glutamine + 8% Foetal Bovine Serum (FBS)

継代と培養方法

Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 3 X 10⁴ cells/cm² Cells should be dislodged by gentle pipetting; 5% CO₂; 37°C.

その他情報

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