CHP-134

6122002, human nerve (neuroblastoma), Neuronal

• 別名: CHP134 Cells, Children′s Hospital of Philadelphia-134 Cells
• UNSPSCコード: 41106514

特性

• 製品名: CHP-134, 06122002
• 由来生物: human nerve (neuroblastoma)
• 成長モード: Adherent
• 核型: Not specified
• 形態: Neuronal
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: metastasis
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human neuroblastoma tumour mass of left adrenal gland

細胞株の説明

A neuroblastoma cell line established from a tumour of the left adrenal area of an 18-month-old male patient. The patient was previously treated with vincristine, cyclophosphamide, dimethyltriazenoimidazole carboxamide, adriamycin, and irradiation. The cells grow as adherent colonies consisting of neuroblast-like cells forming dense mounds. Individual cells radiate from the mounds which reach confluency after routine passaging for several months. This cell line has been reported to have tyrosine hydroxylase activity.

DNAプロファイル

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 9,13
D13S317: 8,11
D16S539: 11,13
D5S818: 11,12
D7S820: 9
THO1: 9,9.3
TPOX: 8
vWA: 18,19

培地

RPMI 1640 + 2mM グルタミン + 10％ ウシ胎児血清（FBS）

継代と培養方法

Split confluent cultures 1:2 to 1:10 every 2-3 days, use gentle agitation to dislodge cells, trypsin/EDTA should not be necessary as cells only adhere loosely; 5% CO₂; 37°C.

その他情報

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