おすすめの製品
リコンビナント
expressed in Streptomyces lividans
品質水準
フォーム
lyophilized solid
比活性
≥18 unit/mg solid
分子量
~37 kDa by SDS-PAGE
保管温度
−20°C
アプリケーション
The enzyme sequence is derived from Nocardia sp., and this cholesterol dehydrogenase is expressed in Streptomyces lividans. Cholesterol Dehydrogenase from Nocardia sp. has been used in the preparation of amperometric cholesterol biosensor for cholesterol detection in biological samples and in a study to investigate the atomic structure of the autosomal recessive hypercholesterolemia phosphotyrosine-binding domain with the LDL-receptor tail. Cholesterol dehydrogenase from Nocardia sp. Has also been used in a study to assess organization, dynamics, and segregation of Ras nanoclusters in membrane domains.
血清コレステロールの定量に用いられる酵素です。
生物化学的／生理学的作用
Cholesterol dehydrogenase (CDH) is a nicotinamide adenine dinucleotide phosphate (NAD(P)) dependent microbial dehydrogenase that oxidizes the 3-β-hydroxyl group of cholesterol to yield cholest-4-ene-3-one. Cholesterol detection by using CDH allows direct and easier quantification compared to cholesterol oxidase based assay.
Cholesterol dehydrogenase is a NAD(P) dependent microbial dehydrogenase that oxidizes the 3-β-hydroxyl group of cholesterol to yield cholest-4-ene-3-one. Molecular mass of the protein is found to be 37 kDa based on SDS-PAGE. Its pH is 4.5.
単位の定義
1 unitは、pH 8.5、25°C、1分間に、1.0 μmolのコレステン-4-エン-3-オンを生成する酵素量です。
物理的形状
凍結乾燥粉末。
シグナルワード
Danger
危険有害性情報
危険有害性の分類
Resp. Sens. 1
保管分類コード
11 - Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
C8743-VAR:
C8743-500UN:
C8743-BULK:
Lorant Janosi et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(21), 8097-8102 (2012-05-09)
Recent experiments have shown that membrane-bound Ras proteins form transient, nanoscale signaling platforms that play a crucial role in high-fidelity signal transmission. However, a detailed characterization of these dynamic proteolipid substructures by high-resolution experimental techniques remains elusive. Here we use
Takeshi Nomura et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(22), 8770-8775 (2012-05-16)
Mechanosensitive (MS) channels of small (MscS) and large (MscL) conductance are the major players in the protection of bacterial cells against hypoosmotic shock. Although a great deal is known about structure and function of these channels, much less is known
Hay Dvir et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(18), 6916-6921 (2012-04-18)
Hypercholesterolemia, high serum cholesterol in the form of LDL, is a major risk factor for atherosclerosis. LDL is mostly degraded in the liver after its cellular internalization with the LDL receptor (LDLR). This clathrin-mediated endocytosis depends on the protein autosomal
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）