C8541

モノクロナール抗サイトケラチンペプチド18 マウス宿主抗体

clone CY-90, ascites fluid

• 別名: モノクロナール抗サイトケラチンペプチド18
• MDL番号: MFCD00162250
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: ascites fluid
• 抗体製品タイプ: primary antibodies
• クローン: CY-90, monoclonal
• 含みます: 15 mM sodium azide
• 化学種の反応性: wide range
• テクニック: indirect immunofluorescence: 1:800 using formalin-fixed, paraffin-embedded sections of human tissue; western blot: suitable
• アイソタイプ: IgG1
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

Cytokeratin 18 (45 kDa) is the primary type I keratin expressed in simple epithelial cells. Epithelial cells and their derivatives characteristically contain intermediate filaments (IFs) composed of about 20 related polypeptides with molecular weights between 40,000-69,000. Each epithelial tissue has a specific and stable pattern of expression of some of these cytokeratin subunits. Epithelium derived tumors maintain the expression of the cytokeratins found in the normal tissue of origin. Therefore, carcinomas can be identified and classified by immunocytochemical staining with antibodies that react specifically with cytokeratins.

Monoclonal Anti-Cytokeratin Peptide 18 (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Cytokeratin 18 (CK18)/keratin 18 is a structural protein and is present in single-layer epithelia. It is located on human chromosome 12q13.

特異性

The antibody reacts specifically with a wide variety of simple epithelia (e.g. intestine, respiratory and urinary systems, liver, and glandular epithelia). It does not react with stratified squamous epithelia (e.g. esophagus or epidermis) or with non-epithelial cells.

免疫原

ヒト表皮癌由来A-431細胞およびヒト乳ガン細胞株MCF-7。

アプリケーション

Monoclonal Anti-Cytokeratin Peptide 18 antibody produced in mouse has been used in:

• immunofluorescence
• fluorescence microscopy
• confocal fluorescence microscopy
• immunolabelling
• histoblots

生物化学的／生理学的作用

Keratins help to maintain structural integrity. They also control Fas?mediated apoptosis and regulate cell size and protein synthesis. Cytokeratin 18 (CK18) plays a major role in the development of lung cancer. It acts as a therapeutic target for non-small cell lung cancer (NSCLC). CK-18 is considered as a promising plasma biomarker to identify patients with HELLP (hemolysis, elevated liver enzymes, low platelets) syndrome.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable