C3787

Chloramphenicol succinate sodium salt

≥80% (HPLC)

• 別名: Chloramphenicol α-succinate
• 化学式: C₁₅H₁₅Cl₂N₂O₈Na
• CAS番号: 982-57-0
• 分子量: 445.18
• EC Number: 213-568-1
• MDL番号: MFCD00079025
• UNSPSCコード: 41116107
• PubChem Substance ID: 24892643
• NACRES: NA.85

特性

• 品質水準: 100
• アッセイ: ≥80% (HPLC)
• 形状: solid
• 溶解性: H₂O: 50 mg/mL
• 抗生物質活性スペクトル: Gram-negative bacteria; Gram-positive bacteria
• 作用機序: protein synthesis | interferes
• SMILES記法: [Na].O[C@@H]([C@@H](COC(=O)CCC(O)=O)NC(=O)C(Cl)Cl)c1ccc(cc1)N(=O)=O
• InChI: 1S/C15H16Cl2N2O8.Na.H/c16-14(17)15(24)18-10(7-27-12(22)6-5-11(20)21)13(23)8-1-3-9(4-2-8)19(25)26;;/h1-4,10,13-14,23H,5-7H2,(H,18,24)(H,20,21);;/t10-,13-;;/m1../s1
• InChI Key: RJOAHMNSYANTPN-OWVUFADGSA-N

詳細

詳細

Chemical structure: phenicole

アプリケーション

Chloramphenicol is often used for bacterial selection in molecular biology applications at 10-20 μg/mL and as a selection agent for transformed cells containing chloramphenicol reistance genes.

生物化学的／生理学的作用

クロラムフェニコールは、細菌タンパク質合成を50Sリボソームサブユニットの23S rRNAと関連しているペプチジルトランスフェラーゼ活性レベルで阻害します。クロラムフェニコール耐性遺伝子を有するトランスフォーム細胞の選択薬として使用されます。

注意

Stock solutions should be stored at 2-8°C and are stable at 37°C for 5 days. Aqueous solutions are neutral and stable over a wide pH range, with 50% hydrolysis occurring after 290 days. Use of a borax buffered solution reduces this number to 14%. Solutions should be protected from light as photochemical decomposition results in a yellowing of the solution. Heating aqueous solutions at 115°C for 30 minutes results in a 10% loss of chloramphenicol.

調製ノート

Stock solutions can be prepared directly in the vial at any recommended concentration. A solution at 50 mg/mL in ethanol yields a clear, very faint, yellow solution. Degradation of chloramphenicol in aqueous solution is catalyzed by general acids and bases. This rate of degradation is independent of the ionic strength and pH.

その他情報

Keep container tightly closed in a dry and well-ventilated place.

安全性情報

• ピクトグラム: GHS08,GHS07
• シグナルワード: Warning
• 危険有害性情報: H302,H351
• 注意書き: P202 - P264 - P280 - P301 + P312 - P308 + P313 - P405
• 危険有害性の分類: Acute Tox. 4 Oral - Carc. 2
• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 個人用保護具 (PPE): Eyeshields, Gloves, type P3 (EN 143) respirator cartridges