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Key Documents

安全性情報

C0871

Sigma-Aldrich

Anti-Cullin 3 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

別名:

Anti-CUL3

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About This Item

MDL番号:
MFCD03454920
UNSPSCコード:
12352203
NACRES:
NA.41

由来生物

rabbit

結合体

unconjugated

抗体製品の状態

affinity isolated antibody

抗体製品タイプ

primary antibodies

クローン

polyclonal

形状

buffered aqueous solution

分子量

antigen ~80 kDa

化学種の反応性

rat, mouse, human

濃度

~1.0 mg/mL

テクニック

immunoprecipitation (IP): 5-10 μg using lysate of rat brain
indirect immunofluorescence: 5-10 μg/mL using mouse 3T3 cells
western blot: 1-2 μg/mL using whole extract of HEK-293T cells expressing human Cullin 3

UniProtアクセッション番号

Q13618

輸送温度

dry ice

保管温度

−20°C

ターゲットの翻訳後修飾

unmodified

遺伝子情報

human ... CUL3(8452)
mouse ... Cul3(26554)
rat ... Cul3(301555)

関連するカテゴリー

詳細

Cullin 3 is a member of the cullin family of E3 ubiquitin protein ligases. Cullin 3 forms the BTB-Cul3-Rbx1 (BCR) ubiquitin ligase complex that contains a RING-box protein, Rbx1, and a BTB containing protein. Rbx1 binds to the C-terminus of cullin 3 through its RING-type zinc finger domain to recruit a ubiquitin-conjugating enzyme. The BTB containing protein binds cullin 3 at its N-terminus and functions as a cullin specific adaptor protein.

アプリケーション

Anti-Cullin 3 antibody produced in rabbit has been used in
  • western blotting
  • immunoprecipitation
  • immunofluorescence

生物化学的／生理学的作用

Cullin 3 (Cul3) acts as a key mediator of post-translational protein regulation. It functions as a scaffold protein that assemble multi-subunit ubiquitin ligase complexes which are responsible for the specific recognition and targeting of substrates for ubiquitin-dependent degradation by the 26S proteasome. Cul3 is required for degradation of the microtubule-severing protein MEI-1/katanin during transitioning from meiosis-to-mitosis phase. It forms a catalytically inactive BTB-Cul3-Rbx1 (BCR) ubiquitin ligase and becomes functional upon covalent attachment of the ubiquitin homologue neural-precursor-cell-expressed. BCR ubiquitin ligase down regulated 8 (Nedd8) near the C terminus of Cul3.

物理的形状

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

10 - Combustible liquids

引火点(°F)

Not applicable

引火点(℃)

Not applicable

個人用保護具 (PPE)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

IXO14807:
C0871-VAR:
C0871-25UL:
C0871-200UL:
C0871-BULK:

試験成績書（COA）

製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Missense variants in RHOBTB2 cause a developmental and epileptic encephalopathy in humans, and altered levels cause neurological defects in Drosophila
Straub J, et al.
American Journal of Human Genetics, 102(1), 44-57 (2018)
Lionel Pintard et al.
Nature, 425(6955), 311-316 (2003-09-19)
Many biological processes, such as development and cell cycle progression are tightly controlled by selective ubiquitin-dependent degradation of key substrates. In this pathway, the E3-ligase recognizes the substrate and targets it for degradation by the 26S proteasome. The SCF (Skp1-Cul1-F-box)
Lionel Pintard et al.
The EMBO journal, 23(8), 1681-1687 (2004-04-09)
Cullin-based E3 ligases target substrates for ubiquitin-dependent degradation by the 26S proteasome. The SCF (Skp1-Cul1-F-box) and ECS (ElonginC-Cul2-SOCS box) complexes are so far the best-characterized cullin-based ligases. Their atomic structure has been solved recently, and several substrates have been described
Wananit Wimuttisuk et al.
Molecular biology of the cell, 18(3), 899-909 (2006-12-29)
Cullins are members of a family of scaffold proteins that assemble multisubunit ubiquitin ligase complexes to confer substrate specificity for the ubiquitination pathway. Cullin3 (Cul3) forms a catalytically inactive BTB-Cul3-Rbx1 (BCR) ubiquitin ligase, which becomes functional upon covalent attachment of
Rebecca Mathew et al.
The Journal of experimental medicine, 211(6), 1137-1151 (2014-05-28)
Induction of Bcl6 (B cell lymphoma 6) is essential for T follicular helper (Tfh) cell differentiation of antigen-stimulated CD4(+) T cells. Intriguingly, we found that Bcl6 was also highly and transiently expressed during the CD4(+)CD8(+) (double positive [DP]) stage of
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