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由来生物
goat
結合体
unconjugated
抗体製品の状態
affinity isolated antibody
抗体製品タイプ
primary antibodies
クローン
polyclonal
フォーム
lyophilized powder
化学種の反応性
human
テクニック
capture ELISA: 0.2-0.8 μg/mL
flow cytometry: 2.5 μg using 106 human T cells treated with PHA
immunocytochemistry: 5-15 μg/mL
western blot: 0.1 μg/mL
不純物
<0.10 EU/μg endotoxin
UniProtアクセッション番号
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... TNFRSF9(3604)
詳細
CD137 (4-1BB) is a member of the tumor necrosis factor (TNF) receptor superfamily. It is a type I transmembrane glycoprotein expressed on the cell surface of activated splenic T cells and thymocytes.
免疫原
NSO細胞で発現させたリコンビナントヒトCD137(4-1BB)の細胞外ドメイン。
アプリケーション
Anti-CD137 antibody produced in goat is suitable for the following applications:
- Agonist Activity: Measured by its ability to costimulate IFNg secretion by human T cells in the presence of anti-CD3. The ED50 for this effect is typically 5-20μg/mL.
- ELISA capture: It can be used as a capturing reagent in a human 4-1BB sandwich immunoassay in combination with biotinylated human 4-1BB detection antibody and recombinant human 4-1BB as the standard. The suggested coating concentration range is 0.2-0.8μg/ml. The recommended concentration of the detection antibody is 0.1-0.4μg/mL.
- Flow cytometry: 2.5μg/106 cells using Human T cells treated with PHA (phytohemagglutinin).
- Immunoblotting: A working concentration of 0.1μg/ml was determined using recombinant human CD137.
- Immunocytochemistry: 5-15μg/mL using immersion fixed human peripheral blood mononuclear cells.
生物化学的／生理学的作用
CD137 in T lymphocytes helps to regulate activation, proliferation and apoptosis. CD137 and CD28 are co-stimulatory molecules of T cell activation. It plays an important role in regulating T-cell-dependent immune responses. The expression of CD137 correlates negatively with lymphocyte proliferation and induces cell death caused by mitogen overstimulation. In monocytes, CD137 induces activation, promotes adherence and helps in prolongs survival.
物理的形状
凍結乾燥品(PBS溶液, 5%トレハロース含有, 0.2 μmフィルター滅菌済み)。
再構成
1 mLの滅菌PBSを用いて0.1 mg/mlに復元してください。
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
C0730-BULK:
C0730-.1MG:
C0730-.1MG-PW:
C0730-VAR:
J Langstein et al.
Journal of immunology (Baltimore, Md. : 1950), 160(5), 2488-2494 (1998-03-14)
CD137 (ILA/4-1BB), a member of the TNF receptor family, was shown previously to inhibit proliferation and to induce apoptosis in T lymphocytes. In this study, we identify CD137 as a novel and potent monocyte activation factor. CD137 protein induces expression
J Langstein et al.
Journal of leukocyte biology, 65(6), 829-833 (1999-06-25)
CD137 (ILA/4-1BB), a member of the tumor necrosis factor (TNF) receptor family, promotes adherence and prolongs survival of human peripheral monocytes. It induces a strong expression of macrophage colony-stimulating factor (M-CSF), an essential monocyte survival factor. Monocyte survival induced by
G Kienzle et al.
International immunology, 12(1), 73-82 (1999-12-23)
Human CD137 is a member of the tumor necrosis factor (TNF) receptor family and the homologue of murine 4-1BB. Recent studies have demonstrated that CD137 promotes accessory T cell activation, and regulates proliferation and survival of T lymphocytes. This study
J Michel et al.
Cytokine, 12(6), 742-746 (2000-06-14)
CD137 is a member of the tumour necrosis factor receptor family which delivers a potent co-stimulatory signal to T lymphocytes. Soluble forms of CD137 (sCD137) can be found at enhanced levels in sera of patients with rheumatoid arthritis. Here we
J C Hurtado et al.
Journal of immunology (Baltimore, Md. : 1950), 155(7), 3360-3367 (1995-10-01)
The expression of the murine T cell Ag 4-1BB, a member of the TNF-R family, is induced by T cell activation. Previously, we and others had shown that signaling through 4-1BB enhanced proliferative T cell responses. To investigate a potential
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