B8895

抗ウサギIgG (全分子)–ビオチン ヤギ宿主抗体

affinity isolated antibody, buffered aqueous solution

• MDL番号: MFCD00162783
• UNSPSCコード: 12352203
• NACRES: NA.46

特性

• 由来生物: goat
• 結合体: biotin conjugate
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: secondary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• テクニック: direct ELISA: 1:200,000; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:800; western blot: 1:400,000-1:800,000 using using an assay detecting actin in total cell extract of HeLa cells (5-10 μg per well)
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

IgG antibodies regulate several functions such as phagocytosis and complement activation. Thus they play a crucial role in facilitating cytological immune responses. Anti-rabbit IgG (whole molecule)–biotin antibody can be used in immunohistochemistry. The product shows minimal interspecies cross reactivity to human IgG hence can be used in enzyme immunoassays or dot blot in presence of human IgG. Anti-rabbit IgG (whole molecule)–biotin antibody reacts specifically to all rabbit immunoglobulin but has no reactivity with human IgG.

IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins
Anti-Rabbit IgG (whole molecule)-Biotin antibody reacts with rabbit serum and rabbit IgG. The product does not cross-react with human IgG.

特異性

全てのウサギIgに結合します。

免疫原

Purified rabbit IgG

アプリケーション

Anti-Rabbit IgG (whole molecule)-Biotin antibody has been used in

• Immunofluorescence
• Immunohistochemistry
• Western blot
• Direct enzyme linked immunosorbent assay (ELISA)

生物化学的／生理学的作用

Avidin, Streptavidin or ExtrAvidine show strong affinity interaction with biotin rendering the biotin-avidin system an extremely effective tool in molecular biology, protein chemistry and immunology. The high specificity of the biotinylated antibody to rabbit IgG and the stability of the biotin-avidin complex has been exploited in variety of reagents (Avidin, Streptavidin or ExtrAvidin conjugated to FITC, TRITC, Peroxidase or Alkaline Phosphatase), for the detection and quantitation of rabbit IgG.

その他情報

ヒトIgG吸収済み抗体です。

物理的形状

0.01M PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)

調製ノート

ヒトサンプルによるバックグラウンド低減のため吸収済みです。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable